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New crystal Structures of Human Glutathione Transferase A1-1 Shed Light on Glutathione Binding and the Conformation of the C-Terminal helix
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Cells were transiently co-transfected with GPCR expression plasmid (50 ng) and the luciferase reporter constructs with cAMP response element (CRE, 200 ng) and TK -Renilla (10 ng) using Lipofectamine 2000 reagent (Life Technologies, Carlsbad, CA, USA) at a ratio of 2:1 (reagent:DNA)
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