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BPC-157: Experimental peptide creates risk for athletes

RNA extraction and qRTPCR analysis Cells were grown to 80% confluency in 75 cm 2 flasks, harvested by trypsinisation, counted using a haemocytometer, and 1 10 7 cells used for RNA extraction using a Qiagen RNeasy mini kit (Qiagen), following the manufacturers protocol for mammalian cells, with additional on column DNAse digestion (RNAse free DNAse kit, Qiagen)