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Although the use of targeted metabolite tracing, such as deuterium tracing of NADPH [7], is successful and remains the gold standard in the field, this approach requires mass-spectrometry, which may not be accessible to many laboratories
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Effect of altered IGF-1R sialylation on its downstream molecules/pathways Since sialylation of the IGF-1R subunit via 2, 6 linkages is important for its conformation and ligand binding 47 , it is of interest to determine whether hyposialylation of IGF-1R affects its downstream signaling events
