sgRNA constructs and gene expressing codon optimized (OPT) plasmid constructs sgRNA control and constructs against CBS and L2HGDH were designed targeting GTATTACTGATATTGGTGGG (sgControl) (#230080, Addgene), GGAGCAGACAACCTACGAGG (sg CBS -1) (#230081, Addgene), TGGCAGTGCTGGCAGCACGG (sg CBS -2) (#230082, Addgene), GATATAGTCATCGTTGGTGG (sg L2HGDH -1) (#230083, Addgene), and CACCAGACTGGACATAACAG (sg L2HGDH -2) (#230084, Addgene) and constructed in lentiCRISPR v2-Blast (#83480, Addgene) for Blasticidin selection at a final concentration of 5 g/ml
I do think the high folate w/ the (absent?) B12 is suspicious
Projections indicate regain plateaus at 75% of lost weight.[4][8][9][2] Physiological Mechanisms Behind Regain The body defends its set point weight through adaptive responses
C., Gustavson, S
Months 24: Building Momentum Toward Steady Weight Loss Between weeks 8 and 16, your semaglutide dose continues to climb toward a therapeutic level, and weight loss typically accelerates to 12 pounds per week for most patients