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Western Blotting To evaluate NGF levels in the striatum and for phosphorylation levels, fresh tissue was homogenized at a 30% ratio (w v 1 ) in kinase extraction buffer A (10 mM HEPES, pH 7.9, 10 mM KCl, 10 mM EDTA, 1 mM DTT, 0.4% v v 1 IGEPAL, 1 mM Na 3 VO 4 , 1 mM PMSF, and 10 mg mL 1 aprotinin and leupeptin) using a previously described method (Kaneko et al., 2004)
Titration is the process of gradually increasing your medication dose over time
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Slowly push the plunger to inject the testosterone
