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In the context of these co-crystal structures, Compound 24 consistently engaged key conserved hydrophobic residues (M336, L388, F392, and F424) and formed hydrogen bonds with Q421, while additional interactions with H223, Y222, I371, H373, I428, P408, L409, M389, and V417 contributed to specificity (Figures 9AE)
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Lastly, genetic manipulation of antioxidant genes, such as Sod1 , Gpx 1 and Cat , in animal models alters susceptibility to alcohol-induced liver injury 28,29,30
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