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The combined effects of increased cytoplasmic monoamine availability and transporter phosphorylation cause reversal of transporter flux, resulting in non-exocytotic neurotransmitter efflux into the synaptic cleftthe characteristic one-two punch mechanism underlying amphetamine-induced monoamine release

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The cells were incubated for 10 min at room temperature with HBSS (basal control), the test compound or the reference agonist and then lysed

On average, patients treated for obesity lost 8.4 % of their original body weight while on the medication, compared with a 4.4 % reduction among those treated for T2DM