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These simulations utilized the lowest-energy solution of the NMR-derived ensemble of the p53 TAD2 -FOXO4-DRI complexes as our starting geometry (see methods)
Johri AM, Heyland DK, Hetu MF, Crawford B, Spence JD
However, these forms were devoid of biological activity
Flow cytometric analyses Apoptosis assay HESC and 12Z cell lines were incubated with NAC, IL-6, TNF-, and IFN- at the doses described in the xCELLigence assay
Protein levels of total ATM and the ROS-activated S1981-phosphorylated ATM were investigated using Western blot analysis
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