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Immunoassay Tear fluid was extracted from half of the remaining strip by agitating small cut pieces of these strips in 100 L PBS, Tween20 0.05% and cOmplete Protease Inhibitor Cocktail (Roche, Basel, Switzerland) at 4 C for 1.5 h 28

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Levels of ThTP and AThTP are higher in fast growing, non-differentiated cells, highlighting the significance of these thiamine derivatives in cellular differentiation (144)

Rogers and Fox 2004) and can promote experimental carcinogenesis of the liver (NTP Toxicology and Carcinogenesis Studies of Theophylline 1998