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RNA samples were treated with the TURBO DNA-free kit (Applied Biosystems, Foster City, CA), further purified through an RNease kit (Qiagen) and quantified with a NanoDrop-1000 (NanoDrop Technologies Inc., Wilmington, DE)

One received lifestyle modifications alone

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As the feeding goes on, hindmilk becomes richer in fat and calories, which helps babies feel full and satisfied

Differential processing of proglucagon by the subtilisin-like prohormone convertases PC2 and PC3 to generate either glucagon or glucagon-like peptide