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We then performed 10,000 bootstrap draws: in each permutation we sampled (with replacement) 11 aquatic nodes from this pool, recalculated the evolutionary rate ( R boot = total novel HG counts divided by total divergence time) and recorded the value, producing a null distribution of novel gene rates in aquatic nodes
Options include once-weekly dulaglutide or once-daily liraglutide, though these medications also carry gastrointestinal side effect risks
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