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Smaller amounts are found in the pancreas, lungs, testis, and thyroid gland
and DNA alkylation, where alkyl groups added to DNA lead to abnormal replication
In vitro barrier models using Caco-2 cells (gut epithelium) or brain endothelial cells could be employed to test the hypothesis that microbiota metabolites, such as butyrateeither administered directly or derived from patient-derived fecal microbiota culturescan strengthen epithelial and endothelial barrier function and reduce oxidative stress [40]
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Serological analysis using ELISA The infection of Helicobacter pylori was confirmed by estimating the levels of antibodies like immunoglobulin G ( IgG) and immunoglobulin M ( IgM) using enzyme-linked immunosorbent assay (ELISA)
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