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Development and validation of test for leaky gut small intestinal and colonic permeability using sugars in healthy adults

Administering vitamin B12 supplements daily, weekly or monthly

Immunofluorescence staining Fibroblasts were fixed, permeabilised, blocked, and incubated overnight at 4 C with primary antibodies targeting p53-pS15 and FOXO4 shown in Table 2, and developed using Alexa Fluor 488-conjugated donkey anti-mouse immunoglobulin G (1:100

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Many dietary supplements contain vitamin B12