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Iron overload induces M1 polarization, as confirmed by studies showing increased M1 markers, such as IL-6, TNF-, and IL-1, with a concurrent reduction in M2 markers, including tissue transglutaminase 2 (TGM2), effectively promoting M1-type macrophage polarization (97)

Secreted frizzled-related protein 2 (sFRP2) functions as a melanogenic stimulator

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For tissue analysis, roughly 25 mg tissue was homogenized in 0.5 mL potassium phosphate buffer

The mitochondrial energy pathway MOTS-c targets responds differently depending on the patients metabolic baseline, and we want to confirm the conditions where you will see meaningful results