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The authors would also like to thank Salvatore Calanna (Novo Nordisk A/S) for review and input into the manuscript

Tryptophan oxidation is the degradation route most commonly detected by RP-HPLC in stored melanocortin analogues, with tyrosine at position 2 and histidine at position 6 secondary sites of concern, and freeze-thaw cycling accelerates both aggregation and oxidative degradation

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The primary endpoint was percentage change in body weight at 24 weeks