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Deep anesthesia was induced using amobarbital sodium administered intraperitoneally at a dose of 150200 mg/kg, in accordance with the approved IACUC protocol and the AVMA Guidelines for the Euthanasia of Animals (AVMA, 2020) [31]

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Realistic skin improvements begin appearing around week 4-6 as the first collagen remodeling cycle completes and continues improving through week 12 and beyond

By enhancing collagen and elastin production in the dermis, they can thicken the skin and increase firmness

Its levels fall with age, so a supervised plan can help rebuild firmness and reduce shedding gradually