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Study characteristics (participant demographics, intervention details, funding sources) were collected, with missing data addressed through author contact and conservative imputation

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The STEP 1 extension study showed that participants who discontinued semaglutide regained approximately two-thirds of their lost weight within one year of stopping treatment

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Finally, using transcription factor motif analysis, we observed that there is more than one binding site motif for ATF4 in the upstream promoter (approximately 1000 bp upstream of the transcription start site) regions of GPX4 , GSS , NFE2L2 , ATG3 , ATG4D , and other genes, and all the sites have the [TGACGTGGC(A/G)] basic motif sequence (Figure 5E)

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