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Gatan K3 direct electron detector positioned post a Gatan BioQuantum energy filter (Gatan, Pleasanton, California, USA), operated in a zero-loss mode with a slit width of 25 eV was used to acquire dose fractionated images of the samples with a 100 m objective aperture

Plasma insulin levels were measured using a mouse insulin ELISA kit (Morinaga, Japan)

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