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2 and plotted the k obs values against the PfGrx C32S/C88S (SSG) or PfGrx C88S (SSG) concentration to determine the second-order rate constants for the formation of roGFP2 WT (S 2 ), roGFP2 C151S (SSG), or roGFP2 C208S (SSG) from the slopes of the linear fits (Fig