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Since superoxide anion cannot diffuse across the inner mitochondrial membrane it remains in the matrix which allows it to react with Fe-S groups in mitochondrial proteins resulting in the liberation of ferrous (Fe 2+ ) iron
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Secondly, the synthesis of glutathione (GSH) is impaired, leading to the functional loss of its dependent antioxidant enzyme, glutathione peroxidase 4 (GPX4)
Generally, antioxidants, also known as inhibitors, act either endogenously, formed within the cells, or exogenously, which are taken from outside the body
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