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This encompasses individuals with obesity, T2DM, and NAFLD, many of whom can have multiple comorbidities

After electrophoresis, the gel was rinsed with distilled water, and to confirm protein separation, it was incubated with Coomassie R-250 reagent for 30 min (1 g L 1 Coomassie R-250, 100 g L 1 glacial acetic acid, 40% (v/v) methanol)

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Some people qualify automatically, such as those with full Medicaid, help from the state for Part B premiums, or Supplemental Security Income