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Each kit includes two 500mg pens (totaling 1,000mg) .You can select doses of 25mg, 50mg, 75mg, or 100mg , with each pen offering five complete 100mg doses . Many individuals adopt cycles such as 25mg daily (five days a week) or 50mg every other day, following a rhythm of 20 days on and 10 days off to maintain robust and balanced NAD+ levels