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For extraction of proteins on cell membrane cell cytoplasm, cell samples were pretreated using a membrane and cytosol protein extraction kit (Beyotime Biotechnology) according to the protocol from manufacture

Nadim MK, Forni LG, Mehta RL, Connor MJ Jr, Liu KD, Ostermann M, et al

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Reducing cholesterol levels : It has been shown to lower LDL cholesterol (bad cholesterol) and triglyceride levels, while simultaneously increasing HDL cholesterol (good cholesterol)

Heinemann L et al