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Defined single-gene and multi-gene deletion mutant collections in Salmonella enterica sv typhimurium
Cultures were pulse-labeled with 10 M BrdU (a nucleoside analog that competes with thymidine for incorporation into DNA) in DMEM-10% HS for the final 2-h period of 48-h in culture, followed by immunocytochemistry for detection of BrdU using G3G4 anti-BrdU mAb (1:100 dilution) and HRP-conjugated anti-mouse IgG Ab (1:500 dilution)
PMC Chang CH, et al
Cerebellar involvement in executive control
However, absence of extensive research on newer variants does not mean they are ineffective, just less studied
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