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In a controlled trial it out-produced both vitamin C and retinoic acid for collagen

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For infection experiments, 3 x 10 8 THP-1 cells were differentiated into macrophages using 20 nM Phorbol 12-Myristate Acetate (PMA, Sigma-Aldrich, UK) overnight, then treated with 50 ng/ml IFN (Immuno Tools, Germany) and 1 g/ml anti-IL10 (BD Biosciences, UK) for one day

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