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Laccase activities were measured using 190 l of reaction mixtures (100 mM citratephosphate buffer pH 4.0 and 1 mM ABTS) in the presence and the absence of a gradient of NaCl (5190 mM) and 10 l of purified LacAnc100 (0.34 g ml 1 ) or LaccID (0.40 g ml 1 ) in flat-bottom 96-well plates (Greiner Bio-One, 655101)
You have a ligand, you have a receptor, and the experiments follow: binding assays, competition curves, functional readouts through a known G protein, receptor-expressing cell lines, selective antagonist controls
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Mol Cell Neurosci 2023;126:103873.ArticlePubMed 74
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