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The section was then incubated with recombinant anti-mucin 5AC antibody (ab3649, Abcam) for 1 h at room temperature, followed by biotinylated secondary antibody (Dako) and HRP detection (HRP conjugated ABC system, Vector Laboratories), according to manufacturers instruction
GSH also exerts its cytoprotective function through conjugation reactions which mediates detoxification of xenobiotics and their metabolites
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Several factors can reduce glutathione levels in the body, including: Chronic stress Poor nutrition Smoking Excessive alcohol consumption Lack of sleep Environmental pollution Aging Certain medical conditions Managing these factors may help support healthy glutathione levels over time
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