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For the solubility test, cell pellet from 250 ml (2 10 12 cells) culture was harvested and 1250 L of a lysis and solubilization buffer containing 50 mM TrisCl pH 7.4, 1 mM EDTA, 10 mM -mercaptoethanol, 1% Triton-X 100, 300 mM NaCl, 1 mM PMSF and 500 L BugBuster Protein Extraction Reagent was added, maintaining the ratio of cells to buffer as ~1.6 10 12 cells/ml of buffer

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