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Pre-chilled LC graded 50% MeOH:H 2 O containing 1 M sulfadimethoxine, as an internal standard, was added to each pVTA sample to obtain a final 1:20 w/v ratio
Such binding may displace other regulatory proteins, making DNA more accessible for transcriptional machinery, potentially inducing neuronal cell differentiation in retinal and periodontal ligament stem cells (hPDLSCs)
Olympus Image Processing Software was used by an investigator who was blinded to the staining to analyse the images
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