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Reversed-phase methods for peptides typically run in acid, most often with trifluoroacetic acid, and acid dissociates this complex

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Alternative reconstitution, with stronger concentration Add 1ml of bacteriostatic water to 5mg vial This creates: 5,000mcg per ml (5mcg per unit on insulin syringe) Smaller injection volume : 250mcg dose = 0.05ml = 5 units 500mcg dose = 0.10ml = 10 units Preferred if you want minimal injection volume