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The following sgRNAs for GGT1 CRISPRCas9 KO were used: sgGGT1 1 (HL-ID: 6) forward: 5-CACCGTGCGGGACGGTGGCTCTG-3
This method is quicker than IV but has slightly slower absorption
The active molecule was identified as the tripeptide glycyl-L-histidyl-L-lysine, and its copper-binding activity was established in subsequent years
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