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The session typically lasts 3060 minutes, depending on the dosage
Then, 20 L of MTT solutions was added to each well followed by an incubation period of 3 hours, then the media were removed and 200 l of DMSO was added to dissolve the crystal and the absorbance was recorded at 570 nm (BioTek, USA) and cell viability was calculated as a percentage compared to cells treated with DMEM alone
The copper uglies occur when chronic GHK-Cu overuse upregulates matrix metalloproteinases (MMPs) past the healthy remodeling threshold
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