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Briefly, 96-well ELISA microplates were coated with HGFR/c-MET Fc chimera (50 ng/well), blocked with 1% BSA, 5% sucrose, and 0.05% sodium azide in PBS, and then incubated for 2 h at 37C with HGF samples

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Social interaction deficits parallel evidence that prenatal cannabinoid exposure disrupts sociability via altered endocannabinoid and oxytocin signaling [54, 55, 56], while broader affective and motivational abnormalities align with persistent synaptic dysregulation in corticolimbic circuits following early-life adversity [57, 58, 59]