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GST fragments with homologous arms were ligated through Exnas II (Vazyme Biotech Co., Ltd., Nanjing, China) into the linearization pcDNA3.1(+) digested by Hind III-HF (Vazyme Biotech Co., Ltd., Nanjing, China)

This triggers a pulsatile release of endogenous growth hormone (GH), which is physiologically important because it mimics the natural GH secretion pattern rather than flooding the body with supraphysiological levels

Certain side effects have been reported in clinical trials, including gastrointestinal discomfort and allergic reactions