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The sections were pre-incubated with 0.5% TNB blocking buffer (PerkinElmer) for 2 hours and incubated with the validated GLP-1 receptor monoclonal antibody (Mab 3F52) at 5 g ml 1 (ref

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Time Zone Adjustments: If you are crossing several time zones, try to stick as closely as possible to your home injection time

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Protein: Many people aim for around 1.5-2.0 g/kg/day to support satiety and preserve lean mass