Total RNA was extracted following the manufacturer's protocol using a spin column-based method (E.Z.N.A Total RNA Kit I, Omega Biotek, Norcross, USA), and its concentration was assessed by measuring absorbance at 260 nm with a Nanodrop spectrophotometer (Thermo Fisher, Waltham, USA)
Dimethyl fumarate increased the activities of SOD, Gpx, CAT, and levels of total thiol (Figure 2)
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Our study found that CTS altered the community structure of gut microbiota and regulated the relative abundance of certain bacterial taxa, including beneficial bacteria such as Firmicutes , Lactobacillus , and Muribaculaceae , as well as potentially harmful bacteria such as Bacteroidota , Pasteurellaceae , Escherichia-Shigella , and Streptococcus