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The plasmid DNA was linearised with the restriction enzyme Spe1 (New England BioLabs, Inc) and transcribed into mRNA by T7 RNA polymerase with the AMBION mMESSAGE mMACHINE TM T7 transcription kit (Invitrogen TM , Thermo Fisher Scientific)
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Many commercial kits also indicate that although 2-Vinylpyridine does not inhibit glutathione reductase, it still interferes with colorimetric glutathione assay
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