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30 Substitution Reactions of L-Carnitine The carboxyl group on L-carnitine can be reduced to a hydroxyl group using reducing agents

It exerts anti-inflammatory effects by modulating TLR4/NF-B signalling pathway and regulation of molecules such as COX-2, iNOS, NF-B and Nrf-2

Mechanistically, it can dampen the pro-inflammatory response by decreasing the transcription of pro-inflammatory cytokines through direct DNA binding to Nuclear factor kappa-light-chain-enhancer of activated B cells (NF-B) (Wang and He, 2022)

As such, it is possible that our results may have been different if samples were in storage for a greater duration of time