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Cells were cultured in RPMI-1640 medium supplemented with 10% heat-inactivated FBS, L-glutamine, penicillin, and streptomycin at 37 C in 5% CO 2

When performed in a licensed medical environment with proper screening, glutathione IV therapy is generally considered safe for suitable candidates

For TLR4-LPS interactions, detailed mechanistic studies have illuminated the discrepancies between in vitro affinity measurements and the physiological responses observed in vivo

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