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After thorough washing in 20 mM HEPES at pH 8, cells were air-dried onto poly-L-lysine (PLL)-coated coverslips and submerged in 70% ethanol at 25 C for 30 minutes for fixation

It is essential to start low and go slow

Optogenetic stimulation of GABA neurons can decrease local neuronal activity while increasing cortical blood flow

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The evolving medication landscape underscores the importance of individualized treatment planning, integrating both pharmacological and behavioral interventions to support long-term recovery across substance categories