When adverse effects occurred, they were typically mild and transient, with the most common being mild to moderate headaches (similar frequency to placebo groups), minor injection site reactions (redness, slight swelling), and gastrointestinal effects at higher oral doses (nausea, diarrhea)
This was to see how GcvA initiates transcription of the glycine biosensor by binding to the gcvBp promoter, how GcvR inhibits GcvA binding to the promoter and how glycine stops GcvR binding to GcvA
In addition to the microbiota itself, intestinal fungi inhibit bacterial colonization by producing alcohol, antibacterial peptides, and some metabolites 145,146
Proc Natl Acad Sci U S A 110(Suppl 2):1039510401
But yeah, I mean, you hear that all the time that people inject something, and there was more in it than they thought