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The alterations in the MPTP lead to swelling, osmotic water influx, and release of pro-apoptotic factors, which can lead to cell death [85]

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Recombinant HGF was treated only with LASSS (at the same 1:8000 molar ratio to HGF as described earlier, without exposure to peroxynitrire) and then applied to centrifugal ultra-filtration (10-kDa MWCO) to thoroughly wash out un-reacted, free LASSS (FW: 238.39) prior to the c-met binding assay