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Western blotting Samples were lysed in RIPA buffer containing a protease inhibitor, and the total protein content was quantified using a bicinchoninic acid protein assay kit (Beyotime)

Eligibility and dosing The tablet is licensed for adults with obesity, with a body mass index (BMI) of 30 or above, or those who are overweight with a BMI between 27 and 30 with at least one weight-related comorbidity, alongside a reduced-calorie diet and increased physical activity

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Hansen et al., 2004a) (Fig