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Approximately 150 g of digested peptide from each sample was used for purification, which is within the C18 spin columns maximum binding capacity of 100 g (#744101, Harvard Apparatus, Holliston, MA, USA) and then lyophilized before being analyzed on the Orbitrap Fusion Tribrid mass spectrometer coupled with an Ultimate 3000 nano-UPLC system (Thermo Fisher Scientific, Waltham, MA, USA)
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