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The plates were incubated for 1 h at room temperature, and after the end of this period, the respective anti-material antibodies conjugated to the biotin were added to the plates and incubated for 1 h at room temperature

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using liraglutide 3 mg/day compared with placebo, despite the lack of changes in total testosterone levels, confirmed the increase in SHBG levels, with a consequent reduction in free Androgen Index (FAI), and also a restoration in menstrual cyclicity [62]

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