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High-sensitivity colloidal Coomassie blue (G-250) staining was performed to examine the purification efficiency
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During autophagosome formation, DHHC7-mediated palmitoylation of autophagy-related protein 16-like protein 1 (ATG16L1) at Cys153 specifically enhances its binding to WD repeat domain phosphoinositide interacting 2B (WIPI2B) and RAB33B, a member of the RAS oncogene family (RAB33B) [15], which ensures efficient recruitment of the microtubule-associated protein 1 light chain 3 (LC3) lipidation machinery to the phagophore [136, 137]
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