Briefly, sections were incubated in 1% cresyl violet solution (OKA, cat# 71041284) at 37 C for 30 min, followed by differentiation in graded ethanol solutions
qPCR was performed to measure the expression level of transcript variants of BDNF, NTRK2, and selected BDNF-coexpressed genes in younger and older subjects
10.1038/s41569-022-00735-4 40 FengX.DuM.LiS.ZhangY.DingJ.WangJ.et al (2023)
Reported side effects Nausea (dose-dependent, more common at higher doses) Facial flushing and increased skin warmth Reduced appetite and mild fatigue Spontaneous erections or increased libido in men Injection site reactions (redness, mild stinging) Changes in existing moles Transient increases in heart rate and blood pressure Storage Lyophilised (unmixed) Freeze at -20 C (-4 F) or below, in dry, dark conditions
If it holds up in larger, biopsy-confirmed studies, hepatic steatosis could become one of the areas where triple agonism has the strongest differentiated rationale though, as with everything about this molecule, that remains to be confirmed in the full program