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The cells were blocked with 5% BSA and then incubated with primary antibodies overnight, followed by incubation with FITC, goat anti-mouse IgG (A22110), DyLight 594, or goat anti-rabbit IgG for 1 h
The supernatant was collected, and the protein concentration was determined with a BCA protein assay kit (Cat
Then, 250 l sample solution (580 and 5800 M in sterile water) and 2.25 ml of culture medium were added into each well in triplicates
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