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The supernatant was concentrated and dried, then reconstituted with 2-chloro-L-phenylalanine (4ppm) solution prepared in 80% methanol-water, finally filtered through a 0.22 m microporous membrane for subsequent analysis

db/db mice (The Jackson Laboratory, 000697) were kept on a chow diet (Brogaarden, Altromin, 1310) and experiments were conducted on 9-week-old male mice

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Applied Nutrition Acetyl L-Carnitine is suitable for vegetarians and vegans and is Halal certified, making it an ideal addition to a balanced diet and healthy lifestyle.