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After overnight culture, the cells were co-transfected with pGloSensor22F cAMP plasmid (Promega) and GLP-1R DNA using Lipofectamine 2000 transfection reagent (Invitrogen)

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This stimulus forces the body to prioritize fat stores for energy instead

The outcome incident or recurrent diagnosis of CUD (International Classification of Diseases, Tenth Revision (ICD-10) code F12 Cannabis related disorders) that occurred within the 12-month time window after the index event) were compared between matched cohorts

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